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Direct Antiglobulin Test (DAT)

Additional Codes

Epic# -

LAB274

 

Cerner name(s):

  • Direct Antiglobulin Test Poly
  • DAT Poly
  • B DAT Poly

Alias: 

  • Direct Coombs

Performing Lab(s)

Memorial-Belleville & Memorial-Shiloh

Methodology

MTS gel or manual tube

Specimen Requirement (s)

Specimen Type: whole blood (WB)

Container/Tube:    Pink (K2EDTA) top (full microtainer is acceptable)

Specimen Volume:    Full tube

Specimen Minimum Volume: 5 mL WB (3 mL for child; full microtainer for infant)

 

Collection Instructions: Must be labeled with lab (barcode) label. 

Day(s)/Shift(s) Test Set Up and Turnaround Time

Sunday through Saturday

Hours: 24 hours

 

Turnaround Time:

STAT: 45 minutes

Routine: less than 1 hours from receipt.

Transport and Stability

Transport temperature: Ambient to lab

 

Analyte Stability: 

Ambient: 4 hours

Refrigerated: 72 hours

Frozen: NA

Reference Values

Polyspecific DAT: Negative

IgG DAT: Negative

Complement (C3) DAT: Negative

CPT/LOINC Codes

86880 (per phase of testing)

Additional Information

This test uses polyspecific AHG for testing. 

  • If negative, no further testing is performed.
  • If positive, IgG and complement will be tested.
    • An eluate will only be ordered and sent to the American Red Cross for testing if one of the following apply:
      • The patient has been transfused with an RBC unit in the past 21 days.
      • The patient has a newly identified alloantibody
      • The patient's history is unknown.

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