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DAT, Infant/Neonate

Important Note

Test can only be run at Memorial - Shiloh.  

 

Test typically performed on cord blood after delivery. 

Test part of cord evaluation which includes ABO/Rh Type, Neonate (less than 4 months of age).

Mother’s information MUST be present on the pink label on the tube under the Cerner label.

Specimen must be labeled with PPID (Cerner) label when computer system is up.  

During downtime, specimen must be hand labeled with

  • patient's name,
  • DOB,
  • hospital registration number,
  • FULL SIGNATURE of phlebotomist or RN drawing the specimen
  • FULL SIGNATURE of witness after verifying patient information and tube
  • date and time specimen was drawn.

Additional Codes

  

Epic# -

LAB274

 

Cerner name(s):

  • DAT IgG Newborn

 

Performing Lab(s)

Memorial-Shiloh only

Specimen Requirement (s)

Specimen Type:  whole blood (WB), cord

Container/Tube:   Pink (K2EDTA) top, lavender (EDTA) top or plain red top

Specimen Volume:    5 mL WB

Specimen Minimum Volume: 1 mL WB

 

Collection Instructions: Must be labeled with baby's lab label.  Mother's information must be completed on the pink label on the tube under the baby's lab label. 

 

Specimen Type: whole blood (WB), venous or capillary

Container/Tube:    Pink (K2EDTA) top, lavender (EDTA) top (full microtainer is acceptable)

Specimen Volume:    2 mL WB

Specimen Minimum Volume: 1 mL WB

 

Collection Instructions: Must be labeled with baby's lab label. 

Unacceptable Conditions

  • Name, gender and DOB on specimen do not match current Cerner record.
  • Hand labeled specimens (unless during downtime)
  • Chart (registration) label used on specimen
  • Grossly hemolyzed.

Day(s)/Shift(s) Test Set Up and Turnaround Time

Sunday through Saturday

Hours: 24 hours

 

Turnaround Time:

STAT: 45 minutes

Routine: less than 2 hours from receipt.

Transport and Stability

Transport temperature: Ambient to lab

 

Analyte Stability: 

Ambient: 8 hours

Refrigerated: 7 days

Frozen: No

Reference Values

DAT IgG: Negative

CPT/LOINC Codes

86880

Interpretive Data

A positive direct antiglobulin test (DAT) IgG in infants/neonates less than 4 months of age could be due to ABO incompatibility with mother or maternal antibodies to red cell antigens or medications which have passed to the infant/neonate.  Before 4 months of age, infants or neonates do not produce their own functioning antibodies and therefore what is found is of maternal origin.  

 

After 4 months of age, orders for a DAT will receive the standard DAT using polyspecific AHG to detect both IgG and complement bound to infant's RBCs.

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