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Test Code MYGLS Myoglobin, Serum

Reporting Name

Myoglobin, S

Useful For

Assessing muscle damage from any cause

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum


Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Specimen Minimum Volume

0.6 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  365 days
  Ambient  7 days

Reference Values

Males: 0 to 72 mcg/L

Females: 0 to 58 mcg/L

Day(s) Performed

Monday through Sunday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

83874

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MYGLS Myoglobin, S 2639-3

 

Result ID Test Result Name Result LOINC Value
MYGLS Myoglobin, S 2639-3

Clinical Information

Myoglobin is a heme protein found in smooth and skeletal muscles. Serum myoglobin reflects a balance between intravascular release of myoglobin from muscle and renal clearance.

 

Previously serum myoglobin had been advocated as a sensitive marker for early acute myocardial injury (eg, acute myocardial infarction: AMI). However, more recent studies indicate that newer markers (eg, troponin) provide superior diagnostic utility in detecting early myocardial injury.

 

Elevation of serum myoglobin may occur as a result of muscle trauma, resuscitation, myopathies, AMI, shock, strenuous body activity, or decreased elimination during renal insufficiency. Extreme elevations occur in rhabdomyolysis. Creatine kinase is released from muscle and used more commonly for this purpose.

Interpretation

Elevated myoglobin levels are seen in conditions of acute muscle injury.

Cautions

Elevation is nonspecific for acute myocardial infarction. The test is of no value in this regard in the presence of kidney failure, rhabdomyolysis, extensive trauma, acute peripheral vascular occlusion, or after seizures.

 

Serum levels rise in renal insufficiency.

 

In very rare cases, gammopathy, in particular type IgM (Waldenstrom macroglobulinemia), may cause unreliable results.

Clinical Reference

1. Lamb EJ, Jones GRD. Kidney functions tests. In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:489

2. Cappenllini MD, Lo SF, Swinkels DW. Hemoglobin, iron, bilirubin. In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:747

Method Description

This myoglobin test is a sandwich-principal assay. The first incubation is 9 mcL of sample, a biotinylated monoclonal myoglobin-specific antibody, and a monoclonal myoglobin-specific antibody labeled with a ruthenium complex, which react to form a sandwich complex. In the second incubation, the complex becomes bound to the solid phase via interaction of biotin and streptavidin after addition of streptavidin-coated microparticles. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Results are determined via a calibration curve, which is instrument specifically generated by 2-point calibration, and a master curve provided via the cobas link.(Package insert: Elecsys Myoglobin. Roche Diagnostics; 10/2022)

Report Available

1 to 2 days

Specimen Retention Time

7 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject

Method Name

Electrochemiluminescent Immunoassay (ECLIA)

Secondary ID

621090