Test Code LAB707 Parathyroid Hormone (PTH) Intact with reflex to PTH Related Peptide (PTHrp)

Performing Laboratory

PTH Intact: Barnes-Jewish Hospital Laboratory

PTHrp: Mayo Clinic Laboratory

BJH Core Laboratory will forward to the performing laboratory. Specimen for PTHrp must be received on wet ice.

Methodology

PTH Intact: Cobas ECLIA electrochemiluminescence

PTHrp: Immunochemiluminometric Assay (ICMA)

Literature References:

Package insert: Parathyroid Hormone. PTH STAT. Roche Corporation 12/2020.

Specimen Requirements

For non hospital patients, call Barnes Jewish Hospital Laboratory Customer Service at 314-362-1470 for STAT pick-up.

Specimen Type: Plasma

Container/Tube: Two Lavender top (EDTA). Place one Lavender top (EDTA) on wet ice for PTHrp testing.

Specimen Volume: Full tube

Day(s) Test Set Up

PTH Intact

Monday through Sunday
Turnaround Time:
STAT: 1 hour after receipt in laboratory
Routine: results available 2-4 hours of receipt

PTHrp

Monday through Thursday

Turnaround Time:

Report availabe 2 to 5 days

Test Classification and CPT Coding

PTH Intact: 83970

PTHrp: 82397

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

Reference Values

PTH Intact: 15-65 pg/mL

PTHrp: ≤4.2 pmol/L

Literature Reference:

Package insert: Parathyroid Hormone. PTH STAT. Roche Corporation 12/2020.

Specimen Transport Temperature

PTH Intact: Send one tube Ambient

PTHrp: Send the other tube on wet ice

Additional Information

For BJH Laboratory Use Only

PTH Intact

Alternate Tube:

1 red-top tube

Note: Serum obtained from clotted whole blood collected with no anticoagulant or preservative (red-top tube) is not preferred and should be avoided if at all possible. It is acceptable if re-collection is not possible.

Laboratory Processing Instructions:

Test performed in BJH Core Lab. Processed by automated line for delivery to the Cobas 8000. Completed specimens are stored refrigerated for 5 days. For PTH gradient, create 2 PTH orders, comment “grafted” and “non-grafted.”

PTHrp

There is no Alternate Tube for PTHrp testing

Laboratory Processing Instructions:

1. Centrifuge specimen in a refrigerated centrifuge or in chilled centrifuge cups.

2. Aliquot plasma into plastic vial and freeze

Laboratory Ordering Instructions:

When ordering a PTH with reflex to PTHRP in the LIS, a Conditional Lavender on Ice must be added on to the order to enable the PTHrp to reflex when the criteria is met.

Call Laboratory Medicine resident at digital pager 314-747-1320 for approval before sending the specimen.

Mayo Test ID
PTHRP

LOINC Code Information

Name	LOINC Code
Parathyroid Hormone Intact, Plasma	2731-8
Parathyroid Hormone Related Peptide	15087-0

Special Instructions